Platelet-dependent thrombography gives a distinct pattern of in vitro thrombin generation after surgery with cardio-pulmonary bypass: potential implications
- Equal contributors
1 Laboratoire d’Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France
2 Inserm U961, Nancy Université, Nancy, France
3 EFS Lorraine Champagne, Nancy, France
4 Haematology Laboratory, CHU Nancy, Rue du Morvan, 54511, Vandoeuvre les Nancy Cedex, France
Thrombosis Journal 2012, 10:15 doi:10.1186/1477-9560-10-15Published: 21 August 2012
Bleeding remains a potentially lethal complication of cardio-pulmonary bypass (CPB) surgery. The purpose of this study was to obtain a better insight into in vitro thrombin generation in the context of CPB.
We used Calibrated Automated Thrombography to assess blood coagulation of 10 low-risk patients operated for valve replacement with CPB, under 2 experimental conditions, one implicating platelets as platelet dysfunction has been described to occur during CPB.
Our main finding was that CPB-induced coagulopathy was differently appreciated depending on the presence or absence of platelets: the decrease in thrombin generation was much less pronounced in their presence (mean endogenous thrombin potential change values before and after CPB were -3.9% in the presence of platelets and -39.6% in their absence).
Our results show that experimental conditions have a profound effect in the study of in vitro thrombin generation in the context of CPB.