|
Comparison of sampling techniques for different coagulation parameters: Vena cava puncture (VCP) vs tail cut (TC) as measured by Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT) in plasma of untreated negative control mice |
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| Assay: |
PT mean (SD) |
aPTT mean (SD) |
number of samples not evaluable |
number of samples analyzed |
% of samples not evaluable |
|
|
|
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| Sampling technique: |
[s] |
[% of standard]* |
[s] |
[n] |
[n] |
[%] |
|
|
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| VCP 1:5 |
9.2 (1.0) |
94 (10) |
31.7 (4.6) |
3 |
15 |
20% |
| VCP 1:10 |
7.8 (0.1) |
116 (13) |
23.2 (0.8) |
0 |
10 |
0% |
| TC 1:5 |
- |
- |
- |
3 |
3 |
100 % |
| TC 1:10 |
- |
- |
- |
4 |
4 |
100 % |
| Ref.values: |
8–10 |
80–120 |
32–36/23–25† |
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|
* where the clotting time of a normal plasma standard was defined as 100% † normal values depended on citrate:blood ratio, and were 32–36 s for 1:5 ratio and 23–25 s for 1:10 ratio | ||||||
Unruh et al. Thrombosis Journal 2005 3:21 doi:10.1186/1477-9560-3-21 |
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