Table 3

Correlation of results of three different assays determining thrombin activity in plasma of mice treated with lipopolysaccharides (LPS) in the presence and absence of the thrombin inhibitor hirudin


Soluble Fibrin determined by agglutination assay
 Soluble Fibrin determined by chromogenic assay
 Thrombin-Antithrombin complexes
Treatment of mice
 sample no.
 semiquantitative result*
 qualitative result (fold increase over negative control)
 qualitative result
 [ng/ml]






LPS, 15 min
 1
 -
 - (1.1)
 -
 3

2
 -
 - (1.3)
 -
 0

3
 -
 - (1.1)
 -
 2

4
 (+)
 + (2.2)
 -
 3

5
 ++
 + (3.7)
 -
 8
LPS, 2 h
 6
 (+)
 - (1.2)
 -
 6

7
 +
 + (2.2)
 +
 21

8
 +
 + (2.2)
 +
 34

9
 ++
 + (2.4)
 +
 20

10
 ++
 + (2.1)
 +
 22
LPS + hirudin, 2 h
 11
 -
 + (2.2)
 -
 10

12
 -
 - (1.6)
 -
 10

13
 -
 - (1.5)
 -
 10

14
 (+)
 - (1.8)
 -
 5

15
 (+)
 - (1.8)
 -
 5
no treatment
 16
 -
 - (1.5)
 -
 2

17
 -
 - (1.1)
 -
 0

18
 -
 - (0.9)
 -
 0

* where ++ corresponds to 0.05 thrombin units/ml, + corresponds to 0.025 thrombin units/ml, (+) stands for borderline result (less than +, but not conclusively -) and – stands for negative (see also Figure 1C).

Unruh et al. Thrombosis Journal 2005 3:21   doi:10.1186/1477-9560-3-21