|
The comparison of methods used for PRP preparation in rabbits |
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| Study |
Rabbit |
Blood Samples |
AC |
Centrifugation |
Hct |
PRP |
Platelets cells Count |
EF |
Activation type |
Adhesive Test |
|||||
|
|
|||||||||||||||
| T |
F |
M |
S |
ml |
Type |
1st |
2nd |
% |
ml |
Vein |
PRP |
% |
|||
|
|
|||||||||||||||
| Efeoglu et al [20] |
23 |
13 |
10 |
Vein |
11 |
EDTA |
300 g/10' |
5000 g/5' |
NS |
NS |
555.000 |
3.134.500 |
465 |
not done |
not done |
|
|
|||||||||||||||
| Butterfield et al [22] |
12 |
0 |
12 |
Ear artery |
21 |
citrate |
150 g/20' |
400 g/10' |
NS |
1 |
468.000 |
2.061.000 |
340 |
CaCl + BT |
not done |
|
|
|||||||||||||||
| Hokugo et al [28] |
15 |
NS |
NS |
NS |
10 |
citrate |
2400 rpm/10' |
3600 rpm/10' |
NS |
0,8 |
200.000 |
1.200.000 |
500 |
Fib + BT |
not done |
|
|
|||||||||||||||
| Ohya et al [29] |
18 |
18 |
0 |
NS |
30 |
NS |
203 g/5' |
1050 g/5' |
NS |
3 |
227.000 |
864.000 |
281 |
CaCl + BT |
not done |
|
|
|||||||||||||||
| Jung et al [30] |
16 |
NS |
NS |
NS |
9 |
citrate |
150 g/20' |
500 g/20' |
NS |
0,5 |
NS |
NS |
NS |
CaCl + BT |
not done |
|
|
|||||||||||||||
| Present study |
40 |
18 |
22 |
Heart |
10 |
citrate |
72 g/15' |
1006 g/5' |
41 |
1 |
320.133 |
807.564 |
152 |
CaCl |
done |
|
T: Total; F: Female; M: Male; Hct: Hematocrit; S: Source; AC: Anticoagulation; EDTA: Ethylenediaminetetraacetic acid; NS: Not Specified; CaCl: calcium chloride; Fib: Fibrinogen; BT: Bovine Thrombin; EF: Enrichment Factor Observation: centrifugation values by Hokugo et al were reported in centrifuge rotor speed (rpm). Not enough data was available to make the conversion to times gravidity (g). | |||||||||||||||
Luengo Gimeno et al. Thrombosis Journal 2006 4:18 doi:10.1186/1477-9560-4-18 |
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